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After 24 h incubation, cells were treated with AuNPs-TMC, free EGFR-siRNA and AuNPs-TMC/EGFR-siRNA (at the concentrations of 100 nM for EGFR-siRNA and 56 g/ml for AuNPs-TMC) and were further incubated for 24 h
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Their unique physicochemical properties enable precise antigen delivery, enhanced immunogenicity, and sustained immune activation, while their versatility allows for the incorporation of multiple antigens and adjuvants into a single formulation
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