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[1] The most commonly used crystalloid fluid is normal saline, a solution of sodium chloride at 0.9% concentration, which is isotonic with blood
doi: 10.1097/00005344-200308000-00016 217 GeorgakisMKvan der LaanSWAsareYMekkeJMHaitjemaSSchoneveldAHet al
The plates were incubated aerobically, lid side up, in humidified plastic boxes at 37 C for 8 h, after which the diameter of the cloud of swimming cells was measured with a ruler
Most research protocols suggest using a reconstituted solution within a few days to a week, though some sources might stretch this
