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M Jefferds, PhD, EIS Officer, CDC

To determine the total glutathione (GSH + 2GSSG) concentration, the following stock solutions were freshly prepared in KPE (0.1 M potassium phosphate, 5 mM EDTA, pH 7.5): 5,5-dithio- bis -2-nitrobenzoic acid (DNTB) at 0.6 mg/mL, NADPH at 0.6 mg/mL and glutathione reductase at 3 units/mL

While this is mostly just what people report from their own experience, it makes a certain amount of sense

We next determined if VRK2 played a role in the GSH metabolic synthesis pathway by regulating one or more of GCLC, GSS, and SLC7A11

Dietary cysteine alleviates sucrose-induced oxidative stress and insulin resistance