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Res., 2014, 36 (3), 276282

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The citric acid cycle appears vulnerable to increases in the NADH/NAD+ ratio, as three NADH sensitive enzymes (-ketoglutarate dehydrogenase, isocitrate dehydrogenase, and citrate synthase) are prone to inhibition by elevations in the NADH/NAD+ ratio (45)

Moreover, the extent of the hydrolysis did increase at basic pH when using DTT ( Figure 4 and Figure S7 )
