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In practice, this likely contributed a small amount of oxygen (from the wet paper towel), as EcN did typically form a biofilm ring at the air-liquid interface under this condition, which was absent for larger-volume biofilm cultures in culture tubes that were incubated strictly anaerobically without humidification

doi: 10.1111/j.1365-2230.2011.04019.x

Together were included: control animals (rats without any surgical intervention, decapitated under anaesthesia) n = 7, rats with ischemia/reperfusion: 3 h n = 5, 24 h n = 7, 72 h n = 5, rats with ischemic preconditioning and ischemia/reperfusion: 3 h n = 5, 24 h n = 7, 72 h n = 5