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Added 50 L diluted serum into the plate, and then added 50 L biotin-labeled CTX-I antibody or CTX-I antibody quickly, followed by incubation for 1 h at 37 C

Fluorescence images were acquired with a Nikon C2 Confocal Microscope A1 (Minato City, Tokyo, Japan) and stitched together using Nikon Elements Software