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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)
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Zinc deficiency affects the thyroid gland by impairing TRH, TSH, T3, and T4 synthesis
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