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Inhibition of TNF-alpha, IL-1alpha, and IL-1beta by Pretreatment of Human Monocyte-Derived Macrophages with Menaquinone-7 and Cell Activation with TLR Agonists In Vitro

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The clarified lysates were loaded onto CAQK-coated Sulfolink-agarose beads (Pierce) and incubated at 4 C for 34 h