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Continuous, daily usage without breaks is generally not recommended due to the lack of extensive human data and potential risks 1 2

This is typically a normal physiological response rather than a direct toxic effect

Lyophilized GHK-Cu maintains potency for 18-24 months or longer when frozen

Topical preparations may use higher dilutions since they don't require injection-compatible volumes

Patients who are low in B12 often notice a difference in as little as 12 hours
