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The fusion constructs were then introduced into yeast AH109 strain using polyethylene glycol/lithium acetate transformation (Clontech), and the growth were assessed of the co-transformed yeast cells on 2D plates (lacking leucine and tryptophan) and 4D plates (lacking leucine, tryptophan, adenine, and histidine)

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Structure-Activity Relationship (SAR) Studies The compound is utilized in structure-activity relationship research to investigate how sequence modifications affect receptor interaction, binding efficiency, and signaling pathway engagement

This is consistent with our previous reports that MMA has potential advantages as a potential prognostic indicator

doi: 10.3389/fmed.2025.1538194 Received 20 December 2024 Accepted 22 April 2025 Published 09 May 2025 Volume 12 - 2025 Edited by Claudia Fiorillo, University of Florence, Italy Reviewed by Sabina Galiniak, University of Rzeszw, Poland Juan Luis Santiago, Poland Universitario de Ciudad Real, Spain Hiroki Satooka, Shiga University of Medical Science, Japan Jie Zhang, Kunming University of Science and Technology, China Updates Copyright 2025 Luo, Hu, Zhou, Zhang, Wu and Sun