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Some of the right lungs were harvested and stored at -80C for subsequent analysis by real time quantitative polymerase chain reaction (qPCR) and western blotting, while other right lungs were perfused with cryomatrix (Thermo, New York, NY, United States) for immunofluorescence staining
IV therapy, by contrast, delivers a larger volume of fluids and multiple nutrients directly into the bloodstream over 30-60 minutes
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Rangon, C.-M
