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Cells were incubated with 0.5 M BODIPY-PAL overnight and immediately, after 6 h or 24 h stained with 0.1 M Nile red (N1142, Thermo Fisher Scientific) or MitoTracker Deep Red (M22426, Thermo Fisher Scientific) for 30 min

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NF1 participants performed below average on every outcome measure at baseline, with the exception of participant six who generated a positive Zscore for dorsiflexion strength and handwriting speed, and participants three and four who also had a positive Zscore for handwriting speed (Figure 2)