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Description
Sirtuin-3 (Sirt3) regulates skeletal muscle metabolism and insulin signaling via altered mitochondrial oxidation and reactive oxygen species production
Hazany S, Hesselink JR, Healy JF, Imbesi SG
Samples were reduced and alkylated using DTT and iodoacetamide respectively, then treated with trypsin and ProteaseMax surfactant 55,66

Proper peptide handling and storage may help reduce degradation pathways such as hydrolysis, oxidation, or structural destabilization during experimental use

Hypertension 64 , 684688 (2014)
