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Peer review Peer review information Nature Reviews Cancer thanks Andrew Teschendorff and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

Two-photon or multiphoton imaging [72] Two-photon imaging (TPI) uses two near-infrared (NIR) photons to excite a fluorophore within the visible range enabling reduction in scattering of tissue background and improving imaging depth

Moreover, chronic kidney disease may occur in convalescent patients

Noise-induced tinnitus can lead to either temporary or permanent tinnitus symptoms, depending on the nature and extent of the damage to the inner ear

These innovations show promise in replacing animal testing, providing reliable alternatives for toxicological research [180, 181]