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The observed masses for the L-iso D-DSIP and D-iso D-DSIP reacted in D 2 O were 2 Da higher (892.4 Da, observed ion: m / z 893.4, z = 1) compared to those reacted in H 2 O (890.4 Da, observed ion: m / z 891.4, z = 1) (Figure S1a,b, respectively), indicating the incorporation of two deuterium atoms likely to the isoAsp and C-terminal Glu residues