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Nikolova, D

The following day, slices were washed with PBS, followed by addition of the secondary antibody for 1 h at room temperature, followed by diaminobenzidine (DAB) solution incubation for 1 min and counterstaining with hematoxylin

Consistent with this idea, protein carbonylation, considered the hallmark for oxidative protein damage and detected using 1,3-dinitrophenol (DNP) flow cytometry, was not increased in G6PDH-null chondrocytes, indicating no manifest increase in oxidative protein damage (Fig

In this study we have analysed the protein expression of over 20 markers of cell cycle, DNA damage, apoptosis, senescence and oxidative stress in hair bulb melanocytes and in the wider growth scalp hair follicle in normal healthy individuals across six decades