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Whether its accidental or intentional, insulin overdose can be a life threatening situation
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Louis, MO, USA) supplemented with 15% fetal bovine serum (Gibco, North Androver, MA, USA), antibiotics: penicillin (10,000 IU/ml), streptomycin (10,000 g/ml), amphotericin B (2.5 g/ml) (Sigma-Aldrich) under the atmosphere of 95% air and 5% CO2 at 37C
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