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Description
10.1093/jn/130.2.496S 147 SanoK.IwasakiY.YamashitaY.IrieK.HosokawaM.SatohK.et al (2021)

Cells were seeded at a density of 1 10 6 cells/mL and were cultured for 24 hours (n = 11) or 72 hours (n = 19) in RPMI-1640 medium supplemented with 10% human serum (Sigma-Aldrich) and penicillin-streptomycin (100 U/g/mL)

Health Canada

The short answer is that the intriguing cell-culture and rodent findings have not been broadly replicated by independent laboratories, the human data are small and old, and Epithalon is not an approved drug anywhere in the West

They must be reconstituted before use.
