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Genes were ranked based on the Wald statistic from the DESeq2 results, and the FGSEA function was executed with 1,000 permutations to identify enriched gene sets

Adherent mouse fibroblast connective tissue cells (L929) and human breast cancer cells (MCF-7 and MDA-MB-231) were obtained from ATCC, Middlesex, UK, and the European Collection of Authenticated Cell Cultures (ECACC), Public Health England, Salisbury, UK, respectively

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Conclusion We conclude that the brains of harp and hooded seals have an overall constitutively enhanced antioxidant defence system, as has been generally found in other tissues of diving mammals