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The metabolic trap hypothesis explores the potential metabolic and immunological mechanisms in ME/CFS, focusing on serotonin and tryptophan metabolism and their effects on patient microbiomes

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Because inflammation is a sensitive health topic, public content should focus on markers, mechanisms, and study design rather than making personal health promises

Incubation time of 510 min was sufficient for optimal reaction with limited degradation

2 Materials and methods 2.1 Ethical standard Under approval number 23-24-23, this study was permitted by the Committee of Health Research Ethics at Qassim University, Kingdom of Saudi Arabia, according to the International Animal Ethics Committee