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Upon its release, Cyt c activates caspase-9 either directly or through APAF-1 in an ATP-dependent manner, resulting in the binding of APAF-1 to caspase-9, a complex known as the apoptosome (6264)

Tissue was homogenized by probe sonication (2 10 pulses, 40% power output), and particulate matter was removed by passing samples through 0.4 m syringe filters

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The inconsistency of the above results may be because the duration of myocardial ischemia in the latter was too short to induce ferroptosis

doi: 10.3168/jds.2013-7454 64 YangQ.CaiY.ZhaoW.WuF.ZhangM.LuoK.et al