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Amplex-type assays report pooled H 2 O 2 and therefore integrate multiple mitochondrial and extra-mitochondrial sites, so accurate source attribution requires site-specific probes (including genetically encoded reporters targeted to matrix, IMS, or outer membrane) and carefully controlled substrate and inhibitor protocols that isolate individual electron-transfer nodes (Waldeck-Weiermair et al

It is a tripeptide, meaning it is made of three amino acids: cysteine, glycine, and glutamic acid

Filipovic A, et al

Gout-associated monosodium urate crystal-induced necrosis is independent of NLRP3 activity but can be suppressed by combined inhibitors for multiple signaling pathways

Neither replaces the other