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Forskningen p GHK-Cu har pgtt sedan 1970-talet och tog fart genom Loren Pickarts tidiga arbete kring kopparbundna peptiders biologiska aktivitet, vilket lade grunden fr mycket av den moderna litteraturen inom omrdet

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Naegelen I, Beaume N, Plancon S, Schenten V, Tschirhart EJ, Brechard S

and (3) the significance of organelle phagocytosis via autophagy

Generation of NEDD8-conjugated CUL1-RBX1 CUL1 neddylation was performed by incubating 8 M CUL1-RBX1, 500 nM APPBP1-UBA3, 1 M UBC12, 10 M NEDD8, and neddylation buffer (50 mM Tris pH 7.6, 100 mM NaCl, 2.5 mM MgCl 2 , 150 uM ATP) at room temperature for 10 min