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Description
The pGADT7 derivatives expressing GAL4-AD fused to either Pmc4 or Cnd2 (GAL4-AD-Pmc4 or GAL4-AD-Cnd2), along with the LEU2 marker gene, were independently transformed into the Mat a Y2HGold budding yeast strain (Clontech)
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It is shown in [14,15] that GSH levels are sensitive to inflammation via multiple mechanisms
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These plant materials undergo solvent extraction and purification to isolate zeaxanthin, often yielding a concentrated oleoresin standardized to 5-20% zeaxanthin
