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Last Updated: May 24, 2026 Introduction to the Steroid Hormones The steroid hormones are all derived from cholesterol

After washing with basis buffer as described before, the column was washed with 25 CV G-buffer OGNG (200 mM NaCl, 20 mM TrisHCl 7.5, 5% glycerol, 0.12% OGNG) and subsequently eluted with G-buffer OGNG containing 400 mM imidazole

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