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WST-1 assay for cell viability and cytotoxicity WST-1 assay ( in-vitro ) was performed by culturing ~3.510 3 cells/well (RWPE-1, DU-145, and PC-3 cells) in a 96-well plate
Additionally, because NAD+ supports metabolic pathways, some practitioners recommend taking a methyl donor supplement (such as TMG, trimethylglycine) concurrently, to aid in processing any excess nicotinamide produced
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Knowing these details helps make a treatment plan that fits you best
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