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The pellet containing the capillaries was suspended in 400 l urea buffer (6 M urea, 10 mM Tris, 1 mM 1,4 dithiothreitol, 5 mM MgCl 2 , 5 mM EDTA-Na 4 , 150 mM NaCl and protease inhibitor cocktail, pH 8.0) and incubated at 4 C over 2 nights

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