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( C ) ESR spectra of DEPMPO-trapped radicals, recorded from HeLa cells expressing APEX2 in either the cytosol (upper left trace) or the mitochondrial matrix (upper right trace), after exposure to HPI (1 mM), H 2 O 2 (1 mM) and DEPMPO (50 mM)

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Following sonication, an additional 4-9 mL of methanol was added, depending on sample size, and the extract rocked overnight at room temperature

Pavan Kumar P, Purbey PK, Sinha CK, Notani D, Limaye A, Jayani RS, Galande S

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