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Substitution of these critical leucine residues to tryptophan eliminated binding affinity, highlighting the importance of maintaining key hydrophobic contacts of leucine side chains, and not general hydrophobicity, which is further exemplified by the evolutionary conservation of leucine residues in LD motifs 41

A proven rotation method involves selecting one primary area per weekfor example, week one uses the lower abdomen, week two uses the right thigh, week three uses the left thigh, and week four uses the upper arm