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Aliquots at the various time points after fecal slurry inoculation were collected and acidified with 2 mL 0.1N HCl (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 g for 8 min, filter-sterilized in a 0.22-m cellulose acetate filter, and frozen at 80 C until performing the experiments for the determination of the amount of TMA in the fecal slurry supernatants

Hydrogen peroxide (H 2 O 2 ) Abcam #ab138874

De Maeyer, R

Vitamin B12 injections are a quick, effective way to support energy levels, cognitive function, and nervous system health

a c , Steady-state concentrations (Conc) of fluorescent compounds in aCSF were calculated by two-photon microscopy above the skull (blue) as well as in the meninges (orange) and brain parenchyma (green) for 1 h after transcranial administration through a surgically thinned (uncompressed) skull