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10.1164/rccm.201809-1651OC 73 SinghD.AgustiA.AnzuetoA.BarnesP

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The hippocampal tissue was disrupted using a T10 Ultraturrax homogenizer (IKA Werke GmbH) followed by RNA extraction with TRIzol Reagent (Invitrogen, Thermo Fisher Scientific) according to the manufacturers instructions

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Multiple patients report that this 3-6 month window is when their spouse first says, "Something's different about you." Blood work at this stage: Your 6-8 week follow-up labs will show whether testosterone levels are in the optimal range, whether estradiol is properly managed, and whether hematocrit, PSA, and other safety markers are stable