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Real-time PCR was performed with SYBR Green Master Mix (Roche, Basel, Switzerland) on a QuantStudio 5 system (Applied Biosystems, Waltham, MA, USA), using the following cycling conditions: 95 C for 10 min, followed by 40 cycles of 95 C for 15 s and 60 C for 1 min

So far, fourteen natural alkaloids have been reported to possess inhibitory potentials towards IKK ( Figure 13-15 ), including berberine ( 43 ), vinpocetine ( 44 ), cryptopleurin ( 45 ), matrine ( 46 ), tetrandrine ( 47 ), piperlongumine ( 48 ), daphnipaxianine A ( 49 ), pipernigamides E-G ( 50-52 ), tulipiferamide A ( 53 ), herbimycin A ( 54 ), himalesine ( 55 ), and ellipticine ( 56 ) [115, 161-169]

Virus-Induced Gene Silencing (VIGS) allows for transitory and sequence-specific knockdown of gene expression, making it a quick and effective way to investigate these candidates