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Description
Lysates were loaded onto NuPAGE 412% Bis-Tris acrylamide gels (1.0 mm, 10-well, Invitrogen), electrophoresed with MES running buffer and then transferred onto 0.45 m PVDF membrane by electroblot using a Mini Blot module (Invitrogen)
Determine the concentration of the controls and samples
Biochem J 1994
Also, the parameter set we analyze contains only influential parameters whose value could not be informed from the literature
Smith R
