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The medium was changed daily, and cells were passaged when they reached confluency using TrypLE Select

GHK-Cu is also shown to stimulate collagen and elastin, smoothing and firming the skin over time without the potential skin irritation of prescription tretinoin or over-the-counter retinol

10.4103/jcrt.JCRT_116_18 J

Urban air pollutants, including particulate matter (PM), ozone (O), and nitrogen dioxide (NO), have been shown to penetrate the stratum corneum and directly induce OS by activating toll-like receptors (TLRs) and the aryl hydrocarbon receptor (AhR) pathway [22]
doi: 10.1038/35003583
