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Likewise, a synthetic gene for mScarlet3 42 carrying the N-terminal Azo-tag directly after the start Met residue (for cytoplasmic expression) was subcloned via the Nde I and Afe I restriction sites

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Rearrangement of (ribose-5-phosphate + xylulose-5-phosphate) to form sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate by transketolase : reversible Glyceraldehyde-3-phosphate is a glycolysis intermediate