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Aliquots (3 L) of ethanolic cell pellet extracts were analysed by means of UPLC-TOF MS using the following solvent gradient for chromatography (0.4 mL/min, 45 C): starting with a mixture (10/90, v/v) of water and methanol, the methanol content was increased to 100% within 8 min and, then, kept constant for 1 min

The fact neurabin is primarily in the brain means that altering its activity should not have the potential body-wide impact of directly altering A1 receptor activity, Wang says