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For comparison of the CMap data to our in vitro studies of the effects of GHK in primary lung fibroblasts, raw data for GHK-treated and control samples were downloaded from the CMap website and normalized using MAS5.0 with the Affymetrix CDF

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According to parabola proportion between inhibition percentage and enzyme activity, different samples have different sample volumes and different sample concentration, you need to determine an optimal sample volume and an optimal sample concentration before measure GPX activity in a new sample