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Description
10.1016/j.jbiotec.2014.12.006
In theory, any enzyme can be evolved using emulsions provided an appropriate substrate that does not diffuse through the emulsion and the desired chemical reaction can be observed for sorting or selection
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10.1016/j.chembiol.2014.01.005
2) Resuspend the cells in 1/10 volume of Lysis Buffer with appropriate amount of protease inhibitors added, which must have no effect on the binding capacity of agarose
