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Flow cytometry analysis Cells treated with the indicated reagents were harvested and stained with Annexin VFITC (BD biosciences, 556419) and 7-AAD (eBioscience, 00-6993-50) for 15 minutes at room temperature in the dark

Consistent with the findings of genetic studies, postmortem surveys have demonstrated lower expression of mRNA associated with GAD and GABA A receptors in individuals with ASD compared to controls [13]

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John Reply Hello David, First I want to thank you for your advice to me previously when I asked whether to go with a younger oncologist which would allow me to see him 3 weeks earlier than an older, more experienced oncologist