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lys2-801 ) with Ent1p and Ent2p endogenously tagged with mNeonGreen were transformed with a vector containing the 5 UTR of Clathrin Heavy Chain, the ORF of CHC1 fused in the 3 with mScarlet (pRS315-scCHC_mScarlet) containing the LEU auxotrophy factor cassette using the following protocol 64

The dipeptide cysteine-glycine is degraded by the enzyme cysteine-glycine dipeptidase found on the cell membrane [50]

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Moreover, long-term exposure to high glucose medium showed an increase of MGO-occludin adducts in BMECs and confirms that hyperglycemia-induced endogenous MGO formation plays a role in tight junction glycation [67]

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