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Complex I activity was assayed kinetically by measuring the Vmax reduction of 2,6-dichlorophenol-indophenol (DCPIP) by spectrophotometer (Specter MAX 190, Molecular Devices, Sunnyvale, CA, USA) [18]
Since the pathways and carbon sources supplying acetyl-CoA in the DKO cells were unknown, DKO cells were cultured in the presence of deuterated water and deuterium incorporation into palmitate was used to examine total de novo lipogenesis