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It was found that a decrease in autophagy promotes lipid accumulation and further inhibits autophagy, increasing lipid retention (Robichaud et al., 2021)

10.1016/j.anaerobe.2016.06.008 114 LeeM.Artola-RecolonsC.Carrasco-LopezC.Martinez-CaballeroS.HesekD.SpinkE.et al

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The construction of the bsh 1 mutagenesis vector was achieved through the following methodology: Strain AR113 served as the template for PCR amplification, employing the primer sets BSH1up-F and BSH1up-R, BSH1down-F and BSH1down-R, and BSH1gRNA-F and BSH1gRNA-R to generate BSH1up, BSH1down, and BSH1gRNA, respectively (Table S1)