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Subsequently, the cells were fixed with 4% paraformaldehyde treated with 0.03% triton X-100 and then blocked with 30% goat serum

Besides the already discussed actions of IGF-1 on glucose, it also has an indirect glucose-lowering effect secondary to its ability in increasing FA oxidation in muscle (will be further discussed in detail within the IGF - 1 and lipid metabolism section)

Bukiri H, Volkmann ER

A., Silva, A., Ap, R., Sanches, P
