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Then, 100 L of media containing 3 M SYTOXTM Green nucleic acid stain (Invitrogen) was added and the cells were incubated for 2 h before fluorescence readings were obtained at 485 Ex /538 Em (Step 1)
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Since pellets are bioidentical and contain hormones like those our body produces, pellets are completely dissolved and absorbed, leaving nothing behind
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1f, purified eIF2B or eIF2B latch tetramers and purified MBP-tagged eIF2B dimers were used to assemble decamers
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