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and 20 mm against Staphylococcus sp., with MIC values ranging from 64 to 128 g/mL across pathogens (Liu et al., 2021)

Sample handling and storage details are summarized in methods section

Biochemistry 60, 259273

Glycerol kinase GK activity was determined as described above for the crude extract measurements with the following modifications: For the continuous PK-LDH assay at 50 C, the assays were performed for Saci_1117 in 0.1 M TRIS-HCl buffer (pH 7 at 50 C), 1 mM MgCl 2 , 1 mM ATP, 0.2 mM NADH, 2 mM PEP, 14 U LDH, 7 U PK using 0.5 g of purified Saci_1117

The honest summary: this isn't a compound with five large randomized controlled trials behind it