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20,000 cells were plated in 100 l medium in a 96-well-plate for 24 h at 37 C

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To block the sEV surface LOX or POSTN, LOX or POSTN blocking antibodies (10 gmL 1 ) in 100 L PBS were used to incubate with sEVs at 4 C overnight, washed with PBS (20 mL), and pelleted by ultracentrifugation to remove non-bound antibodies