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However, when similar degrees of differentiation were accounted for, by aligning differentiating colonies from the termianl differentiation division (TD) and working backwards, we found that the likelihood of a DD division increased over the preterminal divisions, from 0 to 20% at F-5 to 100% at terminal differentiation

High-quality reads containing primer sequences were stitched using Fastq-join 18

Western blot analysis The substantia nigra tissues were homogenized in ice-cold RIPA lysis buffer with 1% protease inhibitor cocktail and centrifuged at 13 000 g and 4 C for 30 min

Autophagy is controlled by several signalling pathways, including the PI3K/Akt/mTOR pathway, the AMPK pathway and the Beclin-1 complex