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The protein concentration was determined spectrophotometrically using the Pierce BCA (bicinchoninic acid) Protein Assay (Thermo Scientific, Rockford, USA)

doi: 10.1016/j.cpcardiol.2023.102155
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It modulates the misfolding and oligomerization of mutant htt exon 1 protein in vitro, reducing polyQ-mediated htt protein aggregation and cytotoxicity

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