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Description
Generally, 1.56 g/L of tea is added to 1 L of boiling water, which is then filtered and steeped with 7090 g/L of sugar [81,82,83]
Visual check: Solution should be clear, colorless (unless B12 is present), and free of visible particles
Important caveat: every claim above comes from cell-culture or animal studies of the individual peptides

A decade of molecular biology of retinoic acid receptors

After that, the sections were incubated with mouse monoclonal GFAP antibody (1 : 500, Sigma) for 2 h at 23C and followed with appropriate goat anti-mouse secondary antibody incubation for 1 h
