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The free amino functional group present on the surface of the membrane was used to covalently couple the first amino acid, and thereafter the remaining amino acids were coupled sequentially to complete the sequences

Cross-linking assay of Sm TG For the BSA cross-linking assay of Sm TG at different reaction times, 40 L of the reaction buffer BD [1.25 mg/mL BSA, 50 M dithiothreitol (DTT)] and 10 L of the 1 Sm TG solution in 100 mM TrisHCl (pH 8.0) were mixed and incubated for 0, 30, 60 and 120 min at 50 C

A comprehensive transcriptomic approach was followed to investigate the expression of susceptible and resistant tomato genes in roots at several infection time intervals in both Meloidogyne incognita susceptible and resistant lines

doi: 10.1016/j.lfs.2006.01.017 117 YangHLiuCNWolfRMRalleMDevSPiersonHet al

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